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Humanized mice using CD34+ from cord blood for investigating malaria parasite
This article describe a new humanized mice model that is a unique tool for investigating, in vivo, the biology of Plasmodium vivax, the most widespread human malaria parasite. This new model of humanized mice using CD34+ cells from cord blood (purchased from Abcell-bio company), allows a reconstitution of the human erythropoiesis.
Conclusion : This model holds promise for understanding in vivo host-parasite interactions and could offer a unique opportunity for in vivo testing of novel antimalarial interventions.
Luiza-Batista C, et al. Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission. Nat Commun. 2022 Jul 15;13(1):4123. doi: 10.1038/s41467-022-31864-6.
Abcell-bio offers an M2 internship position
Abcell-bio offers an M2 internship position for its R&D project on isolated CD34+ cells from cord blood.
Duties: The goal is to optimize and develop new products that would meet the needs of research laboratories. The student will be supervised by the scientific project manager and will work with the laboratory team. He/she will be involved in the design of experiments and will perform the experiments (cell culture, flow cytometry,…). He/she will also analyze the data and be part of discussions around the project. The student will have to follow standard operating procedures according to our quality management system.
Location: Evry (91000), France
Educational level: M2 degree (ongoing) with minimum 12/20 in M1.
Applications to be sent to firstname.lastname@example.org
Risks associated with COVID-19 in pregnancy.
This international cohort study (18 countries) conducted from March to October 2020, evaluated in more than 700 pregnant women with a COVID-19 diagnosis and 1400 pregnant women without a COVID-19 diagnosis, the risks associated with COVID-19 infection on maternal and neonatal morbidity. Women with COVID-19 diagnosis were at higher risk for preeclampsia/eclampsia (relative risk [RR], 1.76; 95% CI, 1.27-2.43), severe infections (RR, 3.38; 95% CI, 1.63-7.01), intensive care unit admission (RR, 5.04; 95% CI, 3.13-8.10), maternal mortality (RR, 22.3; 95% CI, 2.88-172), preterm birth (RR, 1.59; 95% CI, 1.30-1.94), severe neonatal morbidity index (RR, 2.66; 95% CI, 1.69-4.18), and severe perinatal morbidity and mortality index (RR, 2.14; 95% CI, 1.66-2.75). Asymptomatic women with COVID-19 diagnosis remained at higher risk only for maternal morbidity and preeclampsia. Cesarean delivery (RR, 2.15; 95% CI, 1.18-3.91) but not breastfeeding (RR, 1.10; 95% CI, 0.66-1.85) was associated with increased risk for neonatal test positivity.
Conclusions: In this multinational cohort study, COVID-19 in pregnancy was associated with consistent and substantial increases in severe maternal morbidity and mortality and neonatal complications.
Ex vivo expansion of hematopoietic progenitors: interest of a new bioactive peptide.
Although umbilical cord blood (UCB) is a promising source of Hematopoietic stem and progenitor cells (HSPCs), the low dose of HSPCs in these preparations limits their use, prompting need for ex vivo HSPC expansion. This study reports the development of a bioactive peptide named SL-13R, the design of which was originally derived from sequence of the extracellular domain of Delta-like homologue 1 (DLK1) protein, expressed on hepatoblasts. UCB HSPCs were cultured with SL-13R in combination with hematopoietic cytokines under xeno-free conditions. SL-13R enhanced ex vivo expansion of UCB HSPCs without loss of long-term reconstitution ability. Transplantation of cells cultured with SL-13R into immunodeficient NOD/Shi-scid/IL-2R knockout mice confirmed that they possess long-term reconstitution and self-renewal abilities.
As the specialist in the production of hematopoietic progenitors, Abcell-bio is able to provide large quantities of CD34+ cells from a single healthy donor, certifying their purity and viability.
Nii T, Konno K, Matsumoto M, Bhukhai K, Borwornpinyo S, Sakai K, Hongeng S, Sugiyama D. The Bioactive Peptide SL-13R Expands Human Umbilical Cord Blood Hematopoietic Stem and Progenitor Cells In Vitro. Molecules. 2021 Apr 1;26(7):1995. doi: 10.3390/molecules26071995.
Human Umbilical Vein Endothelial Cell Support Bone Formation of Adipose-Derived Stem Cell
This study aims at investigating the co-implantation of human adipose-derived stem cells (ADSCs) after osteogenic differentiation and human umbilical vein endothelial cells (HUVECs) embedded in a vascularized osteogenic matrix of hydroxyapatite (HAp) ceramic for bone tissue engineering. Interestingly, the implantation of HUVEC led to bone formation, suggesting that implanted HUVEC stimulated bone formation. This result may be caused the ability of the extracellular matrix from HUVEC to stimulate the osteogenic differentiation of osteoprogenitor cells. The implantation of osteogenically differentiated ADSC led to higher bone formation than the implantation of HUVEC. But only the co-implantation of ADSC and HUVEC led to a statistically significant larger bone area compared to the other cell-containing groups. This supports the results of earlier studies which showed that endothelial cells stimulate proliferation, cell survival and osteogenic differentiation of osteoprogenitor cells in vitro and bone formation in vivo. Hence, the co-implantation of differentiated ADSCs with HUVECs may therefore be considered as a promising approach for bone tissue engineering. As the specialist in the production of endothelial cells, Abcell-bio is your partner to enable your research.
Winkler S, et al. Support Bone Formation of Adipose-Derived Stem Cell-Loaded and 3D-Printed Osteogenic Matrices in the Arteriovenous Loop Model. Tissue Eng Part A. 2020 Sep 18. doi: 10.1089/ten.TEA.2020.0087.
Abcell-bio is now part of the France Biotech network!
Biotechnology laboratory, Abcell-bio produces quality primary human cells from perinatal tissues. Our team is made up of dedicated scientists, whose objective is to facilitate the R&D work of our customers by forging real partnerships. As such, Abcell-bio is now part of the France Biotech network, whose role is to federate HealthTechs and MedTechs companies in France. The objective is to develop partnerships, private or public, in order to be more and more a player in the ecosystem of health innovation!
Would you like to develop a research partnership? Contact us, the scientific team will be delighted to answer your questions and support you in your projects.
Which are the factors affecting human umbilical cord blood quality before cryopreservation?
Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells (HSCs). Comparatively, UCB HSCs have an extensive proliferative capacity that exceeds that of bone marrow HSCs. However, the small quantity of HSCs obtained from an umbilical cord remains a problematic element restricting the usage of UCB and the quality of UCB units has to be checked before cryopreservation. This study aimed to determine the maternal and neonatal factors that influence UCB before selection for cryopreservation. The analysis included 403 processed UCB units. Neonates with higher birth weights produced better quality UCB units because of increased collected blood volumes, total nucleated cell counts, and CD34+ cell counts. However, an increase in the gestational age from 35 to 41 weeks led to decreases in the collected blood volume and CD34+ cell count.
As the specialist in the production of CD34+ progenitors from cord blood, Abcell-bio controls the collection and the selection of cord blood and guarantees you a number of CD34+ cells for each batch delivered.
Nguyen PH, et al. Factors Affecting Human Umbilical Cord Blood Quality Before Cryopreservation: The Importance of Birth Weight and Gestational Age. Biopreserv Biobank. 2020;18(1):18-24. doi: 10.1089/bio.2019.0063.
Abcell-bio has obtained French Research Tax Credit (CIR) agreement
For several years, Abcell-bio has been committed to its customers in order to facilitate their Research and Development projects. To go further, Abcell-bio has obtained the CIR agreement, which allows French companies to benefit from a tax credit on the expenses engaged in Research & Development or experimental procedures. It has been delivered by the French Ministry of Higher Education, Research & Innovation and it is valid for 3 years, until 2022. The basis for eligibility is an investment in research and development within the European Economic Area.
Please do not hesitate to contact us for any question, our scientific team is at your disposal.
Human CD34 + Hematopoietic Stem Cell-Engrafted NSG Mice: Morphological and Immunophenotypic Features
This study at characterizing the morphological phenotype of NSG mice and their humanized counterparts. CD34+ hu-NSG murine model was generated via perinatal intrahepatic injection of human CD34+ fetal liver cells into NSG mice after preconditioning irradiation. Another purpose of this study was to assess the spectrum of histopathological lesions and identify the main causes of morbidity and mortality in young NSG mice and CD34+ HSC hu-NSG mice. Indeed, despite the increased popularity of these animal models in preclinical research, data on this topic are very sparse. The study describes the most common pathological features in young NSG mice after human hematopoietic stem cell engraftment. Mice frequently showed nephropathy, ovarian atrophy, cataract, and abnormal retinal development, lesions considered secondary to irradiation. In addition, 20% exhibited multisystemic granulomatous inflammatory infiltrates, dominated by human macrophages and T cells, leading to the observed 7% of mortality and morbidity. As some of these lesions contribute to morbidity, morphological assessment of the engraftment at tissue level might help improve immunophenotypic evaluations of this animal model.
Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells.
Widely used for immuno-oncology studies evaluating new therapeutic agents, mice humanized with human tumors (CDX or PDX) and human immune system require carefully selected protocols. This review describes different protocols for establishing humanized mice from peripheral blood mononuclear cells (PBMC) or CD34+ hematopoietic stem cells from cord blood.As the specialist in the production of CD34+ progenitors from cord blood and PBMC, Abcell-bio is your partner to facilitate the production of your humanized mice and significantly enable your research.
Verma B, Wesa A. Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents. Curr Protoc Pharmacol. 2020 Jun;89(1):e77.