Widely used for immuno-oncology studies evaluating new therapeutic agents, mice humanized with human tumors (CDX or PDX) and human immune system require carefully selected protocols. This review describes different protocols for establishing humanized mice from peripheral blood mononuclear cells (PBMC) or CD34+ hematopoietic stem cells from cord blood.As the specialist in the production of CD34+ progenitors from cord blood and PBMC, Abcell-bio is your partner to facilitate the production of your humanized mice and significantly enable your research.