Frozen Human Umbilical Artery Endothelial Cells (HUAECs)

Category

Endothelial Cells

Characteristics

Passage 2 or 3 — Passage 1 on request — Single Donor

Applications

Angiogenesis — Inflammation — Oncology — Vascular Biology
Isolated human umbilical artery endothelial cells (HUAEC) 500K P2
Passage after thawing P2, 500,000 cells
Ref. 2003-5002
Isolated human umbilical artery endothelial cells (HUAEC) 500K P3
Passage after thawing P3, 500,000 cells
Ref. 2003-5003

Human Umbilical Artery Endothelial Cells (HUAECs)

Umbilical Cord

Human Umbilical Artery Endothelial Cells (HUAECs) are mature human endothelial cells isolated from the umbilical cord artery. The specific markers of endothelial cells, CD31, CD144 and KDR surface antigens, are expressed on these adherent cells. These cells present a typical cobblestone morphology. HUAECs offer a tool to study angiogenesis, inflammation or atherosclerosis as well as providing an in vitro model for pharmacology studies.

Product description

Abcell-bio offers mature endothelial cells isolated from the human umbilical cord artery.

Based on their arterial origin, HUAECs specifically express markers, such as EphB2, neuropilin-1, Notch 3 receptor, and Notch Delta-like4 Ligand, which are not expressed by endothelial cells from the umbilical cord vein (HUVEC). Our HUAECs are derived from a single, healthy donor.

Quality control

HUAEC cells are tested for the specific markers of endothelial cells, such as CD31, CD144, KDR and vWF. The expression of these markers is analyzed by Flow Cytometry and Immunohistochemical Labeling. Abcell-bio evaluates the specific endothelial functions of each HUAEC batch, such as the ability to incorporate acetylated LDL or the potential expression of activation molecules (ICAM-1, VCAM-1). These cells are able to form capillary-like structures on Matrigel® reflecting their angiogenic capacity. HUAECs produced by Abcell-bio are cryopreserved in FBS-10% DMSO and provided at passage 3 after thawing (passage 2 on request). Virologic (HIV1/2, HBV, HCV) and mycoplasma detection tests are performed on all batches. A full certificate of analysis is provided for each batch of HUAEC cells ordered.

Order customization on request

Early passage, specific batch size