HUAEC cells are tested for the specific markers of endothelial cells, such as CD31, CD144, KDR and vWF. The expression of these markers is analyzed by Flow Cytometry and Immunohistochemical Labeling. Abcell-bio evaluates the specific endothelial functions of each HUAEC batch, such as the ability to incorporate acetylated LDL or the potential expression of activation molecules (ICAM-1, VCAM-1). These cells are able to form capillary-like structures on Matrigel® reflecting their angiogenic capacity. HUAECs produced by Abcell-bio are cryopreserved in FBS-10% DMSO and provided at passage 3 after thawing (passage 2 on request). Virologic (HIV1/2, HBV, HCV) and mycoplasma detection tests are performed on all batches. A full certificate of analysis is provided for each batch of HUAEC cells ordered.